FluoGen: An Open-Source Generative Foundation Model for Fluorescence Microscopy Image Enhancement and Analysis

University of Science and Technology of China
2026
FluoGen, a diffusion-based generative foundation model.

We present FluoGen, a diffusion-based generative foundation model designed to improve the performance of AI-based methods in data-constrained fluorescence image processing.

Super-Resolution

Qualitative results of our FluoGen-s method. Drag the slider to compare the low-resolution input (Before) with the high-resolution output generated by FluoGen (After).

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Image Denoising

Qualitative results of our FluoGen-n method. Our method effectively removes noise while preserving fine structural details.

Segmentation

FluoGen generates realistic images from segmentation masks, serving as a powerful data augmentation tool for segmentation methods.

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Large-scale, heterogeneous fluorescence microscopy dataset for generative pre-training.

Large-scale, heterogeneous fluorescence microscopy dataset for generative pre-training. The scale and diversity of the dataset provide a robust foundation for learning generalizable representations of cellular organization.

Qualitative comparison of FluoGen with other generative methods on representative cellular structures.

Visual comparison of super-resolution results from FluoGen-s and other state-of-the-art methods on typical organelles (F-actin, microtubules, ER, and CCPs) from the BioSR dataset. Corresponding ground truth (GT) images are shown for reference.